ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Objective To evaluate the application of black box method in mosquito surveillance by comparing the results from black box and lamp trap methods. Methods Two lamps were set at each surveillance site and one black box was put at a 10 m interval from each lamp. Surveillance was conducted twice every month. Results The total number of captured mosquitoes and the number of female mosquitoes by the lamp trap method was 3.50 times and 3.75 times higer than those monitored by black box method, respectively. The seasonal fluctuation curves reflected by both Methods were unimodal, with a peak in July. The monthly calculations of mosquitoes of the two Methods were highly correlated. Mosquitoes captured by the black box method included Culex pipens pallens (84.64%), Cx. tritaeriorhynchus (10.02%), and Anopheles sinensis (3.34%), and those by the lamp trap method were Cx. pipens pallens (80.47%), Cx. tritaeriorhynchus (11.79%), and An. sinensis (6.88%). Only a few Aedes albopictus and Armigeres subalbatus were trapped. The male-female ratios were similar between the two methods, whereas great difference in the mosquito number was found in distinct environments. Black box method was more effective in parks and hospitals than the other, while lamp trap was more effective in pasture lands and farms. Two peaks of mosquito density were shown on 05: 00 and 19: 00 with environmental difference between day and night. The day and night peaks of density were also present in parks, farms and pasture lands except hospitals and residential areas where only one night peak was shown. Conclusion With fewer requirements for equipment, operation skills and power supply, the black box method is an effective alternative to the lamp trap approach. Deployment before the night peak of density and collection after the early morning peak is recommended for mosquito monitoring.
Objective To analyze the species composition, seasonal variation and the diversity of fly at different habitants in Hongkou district, Shanghai from 2006 to 2010 to provide evidence for the prevention and control. Methods Cage traps with sugar and vinegar baits were used to capture flies. Quarterly analysis of biodiversity was performed from March to November each year. Results The flies in this district belonged to 10 species, 7 genera and 3 families. The highest density was noted in Musca domestica (0.62 flies per day for every cage). The density of M. domestica was increased, whereas the density of Lucilia sericata declined each year. The fly densities were 2.52, 1.91, 1.46, 1.12, and 0.70 flies per day for every cage from 2006 to 2010, respectively. The highest density was noted in late April, early May, late June and mid-July from 2006 to 2010. In July, eight species were collected at most. The species diversity index and evenness index were highest in May. Different habitats by fly density in descending in the last five years were: farm trade market > residential area > public green belt > external environment of restaurants. The most abundant species were observed in residential areas (ten species), which had the highest species diversity index and evenness index. Conclusion The fly diversity declined year by year from 2006 to 2010, which had a peak from late April to mid-July each year. The dominant species were M. domestica and L. sericata. The population characteristics could be generally described by the species diversity index and evenness index.
Objective To compare mosquito densities using the black box method and the carbon dioxide method and explore the application of the black box approach. Methods The black box method and the carbon dioxide method were used to measure mosquito density and composition at seven locations in two districts of Shanghai. Correlations between species composition, sex ratios and seasonal variation were compared using the two Methods. Results The average mosquito density using the black box method (25.63 mosquitoes/box) was 3.50 times higher than the carbon dioxide method (7.32 mosquitoes/ device), and the average density of female mosquitoes using the black box method (14.65 mosquitoes/box) was 2.35 times higher than the carbon dioxide method (6.23 mosquitoes/device), densities were significantly different. The density indices of the two approaches were not correlated. The species composition recorded by the black box method was: Culex pipiens pallens (94.97%), Aedes albopictus (4.97%), Cx. tritaeriorhynchus (0.03%), and Anopheles sinensis (0.03%). The species composition recorded by the carbon dioxide method was: Cx. pipiens pallens (79.26%), Ae. albopictus (18.27%), Cx. tritaeriorhynchus (2.10%) and An. sinensis (0.37%). The differences in composition were also statistically significant. Conclusion As compared to the carbon dioxide method, the black box method is readily applicable, simple, affordable and can be used in most locations, particularly habitats dominated by Cx. pipiens pallens. The carbon dioxide method can be used for the monitoring of both Cx. pipiens pallens and Ae. albopictus.
Objective To explore the on-site fly control effects of a new biological agent, Echo, in animal farms. Methods Echo, chlorbenzuron, pyriproxyfen, a mixture of Echo with chlorbenzuron, and a blank control were sprayed at the same concentration onto feces in a laboratory simulation and in the field. Results After treatment with Echo for 12 days, compared to the control group, the emergence rate of flies from the feces exposed at 24 h, 48 h and 72 h, decreased by 96.2%, 64.4% and 86.7%, respectively. After treatment with Echo and the mixture of Echo and chlorbenzuron for 7 days and 12 days in the field, the density of fly larvae decreased by 56.2% and 65.5%, respectively. After treatment with Echo for 12 days, fly density decreased by 71.2% compared to the control. After treatment with the Echo and chlorbenzuron mixture for 12 days, fly density decreased by 55.2% compared to the control. Fly density also decreased by 74.5% after treatment with Echo for 6 h in the field. Conclusion The Results show that Echo was effective in deodorization, inhibition of fly breeding and reduction of fly density.
Objective To compare the black box method with the labor hour method in terms of their differences in the surveillance of mosquito density and explore the possibility of substituting black box method for the labor hour method for the surveillance. Methods The differences between the black box method and the labor hour method in terms of their performance in the surveillance of mosquito density and species were compared based on the data from three independent surveillance sites, with the correlation of the two Methods in the surveillance of the species composition ratio, sex ratio and seasonal fluctuation trends examined. Results The Results showed that the density of mosquito by black box method was 1.51 mosquitoes per site, lower than that by labor hour method (3.13 mosquito per box), with a significant difference between them (t=4.985, P=0.0025). In terms of the seasonal fluctuation tendency of mosquitoes, similar findings from the two Methods were obtained with good correlation and significant statistical difference (r=0.8833, P=0.0084). It was found that the density of female mosquitoes by the labor hour method was 1.88 mosquitoes per site, higher than that by black box method (0.50 mosquito per box) with a significant difference between them (t=5.043, P=0.0023). There was no correlation between the fluctuation curves of the two methods(r=0.4812, P=0.2743). The species and composition of mosquitoes were also different with the two different methods. The main species collected by the black box method was Culex pipiens pallens(90.6%), followed by Cx.tritaeniorhynchus(4.1%), Aedes albopictus (2.4%) and Armigeres subalbatus(2.9%), with no Anopheles sinensis found. As far as the labor hour method was concerned, Cx. pipiens pallens(42.8%) and Ar. subalbatus (40.3%) were the main species collected, with Cx. tritaeriorhynchus, Ae. albopictus and An. sinensis accounting for 11.7%, 4.7% and 0.5% respectively. In addition, there was a different sex ratio of the mosquitoes collected with the two different methods, with the number of male mosquitoes larger than that of female ones in the surveillance with the black box method, and the opposite being true for the labor hour method. The quantity of female mosquitoes collected by the black box method was close to that by labor hour method, but there was a statistically significant difference (χ2=146.18, P<0.001). The quantity of male mosquitoes collected by the black box method was larger than that by the labor hour method with a statistically significant difference (χ2=513.01, P<0.0001). Conclusion The black box method, capable of capturing more female than male mosqitoes, is applicable to the surveillance of a variety of mosquito species , but the regularity and standard operating procedure (SOP) of the method as a surveillance tool remains to be studied.
Objective To evaluate the effectiveness of 0.5% pyriproxyfen granules for control of Aedes albopictus, providing the basis for mosquito control drugs and techniques during the Shanghai World Expo. Methods Larvae immersion was conducted to determine the mortality, pupation and eclosion rates at different concentrations under laboratory conditions and simulated field conditions. The control effects at 0.06 and 0.08 mg/L on different larval instars were assessed. Pyriproxyfen was applied on-site to the breeding sites of Ae. albopictus (water wells and other water bodies) in residential areas, and the mosquito density was measured using the mosquito biting method. The larvae-containing water bodies were collected from the experiment field for further observation of larval growth and development. Results (1)At the concentration gradient from 0.06 to 0.12 mg/L, the mortality of larvae was 25.75%-49.00% and the pupation rate 31.19%-40.25% under laboratory conditions; adult mosquitoes did not emerge. The pupation rate of the control group was 99.25%, and the eclosion rate was 95.47%;(2)the tested agent inhibited the pupation and eclosion of multiple larval instars at 0.06 and 0.08 mg/L, and the eclosion rate decreased as the concentration rose; (3) under the simulated environment, pyriproxyfen effectively inhibited the pupation and eclosion of mosquitoes at a concentration of above 0.5 mg/L in the shade; and(4)the density of both larval and adult mosquitoes reduced sharply at the breeding sites after on-site application of pyriproxyfen granules, according to the investigation of breeding sites and adult mosquito biting method. Only 0.24%-0.71% of the larvae collected from the water bodies developed into adults. Conclusion Pyriproxyfen can inhibit the growth and development of Ae. albopictus larvae. 1 mg/L of pyriproxyfen can be directly applied to the breeding sites for mosquito control.
Disease vectors are basically organisms that transmit pathogens of various diseases. Shanghai World Expo is to be held during the peak period of disease vectors. Therefore, to reduce densities of disease vectors and lessen the threat of vector?borne diseases has become an important part of public health protection during the Expo. An expert consultation was convened to elaborate issues on the control of disease vectors throughout the Expo, and expert advice was adopted by relevant departments. In view of this, inspection of disease vectors in various industries of the city is further strengthened; technical documents of control measures applicable for the Expo are developed, and emergency response training and drills are improved for the professional and technical personnel of vector control. As a result, a solid foundation has been laid for public health protection, particularly the control of vectors, during the Expo.
Objective To evaluate the effectiveness and comparability of three methods of cockroach density monitoring in the same unit, providing scientific basis for the standardization of cockroach density monitoring and the assessment of control efficacy. Methods Three commonly used density monitoring and measurement approaches were tested in three units with different infestation rates. For each place, visual observation, glue traps and sprays were concurrently conducted in order. Data statistics was tested using Chi-square, two-sample mean-comparison test and one-way ANOVA. Results (1) Except for three units where no cockroaches were captured, cockroaches were detected in 28 out of 31 experimental units by the three monitoring methods. (2) The detection rates of visual observation, glue traps and sprays were 48.82%, 46.08% and 48.44%, respectively, without statistically significant difference (χ2=0.556,P≥0.05). The density indexes were 9.65 /room, 7.04 /trap and 13.57 /room, respectively.(3) Regardless of high, intermediate or low infestation rates, no statistical difference was shown in the positive rates and density indexes derived from three approaches. Conclusion The three methods were all useful in density monitoring, investigation and efficacy assessment. However, the sample size, the responsibility of monitors and the adherence to standard procedures determine the accuracy and reliability of positive rates and density indexes whichever approach is taken. Establishment of indexes for glue traps is desired.
【Abstract】 Objective To verify the effect of simvastatin on the population dynamics of Blattella germanica. Methods Two groups of B.germanica were reared with feedstuff containing 0.001% simvastatin and common feed, respectively. The population quantity of different development stages of B.germanica and their constitution ratio were investigate after breeding for different time. Results The quantity of adult cockroaches with oothecae in the tested group reduced gradually after treatment for two months, six months and eight months, respectively. Accordingly, the quantity of male and female adult cockroach also decreased gradually. The nymphae quantity after treated for four months was two times than that after treatment for two months. However, it decreased rapidly in the sixth month, and the quantity of female adults and adults with oothecae reduced to zero in the eighth month. The quantity of male adults and nymphae was 2 and 15, respectively. The quantity of adults with oothscae was all higher than the initial number after they were bred with common feed for two months and six months. But in the forth month, it decreased a little compared to the initial number. And, it was basically equal to the initial number in the eighth month. With the decrease of the adults with oothecae, the quantity of male and female adults also reduced gradually. Similar to the tested group, the nymphae quantity in the control group was the highest after breeding for four month, and then it gradually decreased. In the eighth month, there were 78 nymphae, 5 adult cockroaches with oothacae, 8 female adults and 12 male adults. Conclusion B.germanica would lose the capability of its population restoration after breeding with feed containing 0.001% simvastatin for eighth months.
【Abstract】 Objective To compare biological characteristics and population dynamics of deltamethirn-resistance strain and susceptible strain of Aedes albopictus in the laboratory. Methods Biological characteristics of two mosquito strains such as reproduction, development and bloodsucking were observed and recorded in the laboratory, and the life tables of them were established. Results The hatching rate, pupation rate and emergence rate of resistance strain were lower than that of susceptible strains, decreasing about 16.67%, 8.92%, and 0.44% respectively, and the difference of them were significant(P<0.05). The bloodsucking rate and the quantity of female filial generation reproduced by each female of resistance strain were also lower than that of susceptible strain. R0 and rm of resistance strain were 151.86 and 0.16 respectively, however that of susceptible strain were 177.18 and 0.20. Conclusion The resistance of Ae.albopictus to deltamethrin results in the decrease of adaptability to environment change and the disadvantage of reproduction.